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Image Search Results
Journal: Development (Cambridge, England)
Article Title: Revising the embryonic origin of thyroid C cells in mice and humans
doi: 10.1242/dev.126581
Figure Lengend Snippet: Tracing Sox17 + progeny in embryonic thyroid in Sox17-2A-iCre;R26R mice. Immunofluorescence images of single and merged channels of the indicated markers from serial sections of the same specimen; merged images additionally show 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain. (A) Coexpression of β-galactosidase (β-gal) and Nkx2-1 in follicular cells (upper panel), β-gal and CD31 in endothelial cells (middle panel), and β-gal and calcitonin in C cells (lower panel). (B) Colocalization of β-gal and calcitonin as revealed by confocal laser scan microscopy. Arrows indicate β-gal+ C cells. Arrowheads indicate β-gal– stromal cells. Scale bars: 100 µm (A); 25 µm (B).
Article Snippet: Cryosections (10 µm thick) collected on glass slides were subjected to pairwise double immunolabeling as indicated with the following antibodies: rabbit anti-Nkx2-1 (Biopat, Italy; PA0100, 1:1000), rabbit anti-calcitonin (DAKO; A0576, 1:500), guinea pig anti-Foxa1 (kindly provided by Jeffrey Whitsett, Cincinnati Children's Hospital, OH, USA; 1:2000; applied for mouse tissue only), mouse anti-Foxa1 (Seven Hills Bioreagents; WMAB-2F83, 1:1000; applied for human specimens only), rabbit anti-Foxa2 (Seven Hills; WRAB-FOXA2, 1:2000), rat anti E-cadherin (ECCD2; Calbiochem; 205604, 1:1000),
Techniques: Immunofluorescence, Staining, Microscopy